This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV5B-Flag-Smad3
catalog :
11742
citations: 13
Reference
Jeon H, Pornour M, Ryu H, Khadka S, Xu R, Jang J, et al. SMAD3 promotes expression and activity of the androgen receptor in prostate cancer. Nucleic Acids Res. 2023;51:2655-2670 pubmed publisher
Xie X, Urabe G, Marcho L, Williams C, Guo L, Kent K. Smad3 Regulates Neuropilin 2 Transcription by Binding to its 5' Untranslated Region. J Am Heart Assoc. 2020;9:e015487 pubmed publisher
Cibi D, Mia M, Guna Shekeran S, Yun L, Sandireddy R, Gupta P, et al. Neural crest-specific deletion of Rbfox2 in mice leads to craniofacial abnormalities including cleft palate. elife. 2019;8: pubmed publisher
Sato F, Otsuka T, Kohsaka A, Le H, Bhawal U, Muragaki Y. Smad3 Suppresses Epithelial Cell Migration and Proliferation Via the Clock Gene Dec1, Which Negatively Regulates the Expression of Clock Genes Dec2 and Per1. Am J Pathol. 2019;: pubmed publisher
Wang Y, Zhang X, Huang H, Xia Y, Yao Y, Mak A, et al. Osteocalcin expressing cells from tendon sheaths in mice contribute to tendon repair by activating Hedgehog signaling. elife. 2017;6: pubmed publisher
Kim G, Lim H, Park H. Binding of coronin 1B to T?RI negatively regulates the TGF?1 signaling pathway. Biochem Biophys Res Commun. 2017;490:574-579 pubmed publisher
Manresa M, Tambuwala M, Radhakrishnan P, Harnoss J, Brown E, Cavadas M, et al. Hydroxylase inhibition regulates inflammation-induced intestinal fibrosis through the suppression of ERK-mediated TGF-?1 signaling. [corrected]. Am J Physiol Gastrointest Liver Physiol. 2016;311:G1076-G1090 pubmed publisher
Kong Y, Zou S, Yang F, Xu X, Bu W, Jia J, et al. RUNX3-mediated up-regulation of miR-29b suppresses the proliferation and migration of gastric cancer cells by targeting KDM2A. Cancer Lett. 2016;381:138-48 pubmed publisher
Tian Y, Yuan W, Li J, Wang H, Hunt M, Liu C, et al. TGFβ regulates Galectin-3 expression through canonical Smad3 signaling pathway in nucleus pulposus cells: implications in intervertebral disc degeneration. Matrix Biol. 2016;50:39-52 pubmed publisher
Bollinger L, Witczak C, Houmard J, Brault J. SMAD3 augments FoxO3-induced MuRF-1 promoter activity in a DNA-binding-dependent manner. Am J Physiol Cell Physiol. 2014;307:C278-87 pubmed publisher
Ferguson B, Gao X, Zelazowski M, Lee J, Jeter C, Abba M, et al. The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding. BMC Cancer. 2013;13:593 pubmed publisher
Kim S, Lim J, Woo C. ERK5 inhibition ameliorates pulmonary fibrosis via regulating Smad3 acetylation. Am J Pathol. 2013;183:1758-1768 pubmed publisher
Labbe E, Silvestri C, Hoodless P, Wrana J, Attisano L. Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein FAST2. Mol Cell. 1998;2:109-20 pubmed
product information
Catalog Number :
11742
Product Name :
pCMV5B-Flag-Smad3
article :
doi10.1016/S1097-2765(00)80119-7
id651
pubmed_id9702197
bacterial resistance :
Ampicillin
cloning :
backbonepCMV5
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5ClaI
promoter
sequencing_primer_3hGH-pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
geneSMAD3
id4088
genbank_ids
NM_005902
mutation
nameSMAD3
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
plasmid copy :
R. Derynk, UCSF
resistance markers :
589
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA