This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMXs GFP-LC3-RFP
catalog :
117413
citations: 6
Reference
Xue R, Fan X, Yang Q, Yu C, Lu T, Wan G. Protective effect of ethyl ferulate against hypoxic injury in retinal cells and retinal neovascularization in an oxygen-induced retinopathy model. Phytomedicine. 2023;121:155097 pubmed publisher
Paul S, Dansithong W, Gandelman M, Figueroa K, Zu T, Ranum L, et al. Staufen Impairs Autophagy in Neurodegeneration. Ann Neurol. 2022;: pubmed publisher
Xiao W, Wang J, Wang X, Cai S, Guo Y, Ye L, et al. Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy. 2022;:1-21 pubmed publisher
Deng J, Thennavan A, Dolgalev I, Chen T, Li J, Marzio A, et al. ULK1 inhibition overcomes compromised antigen presentation and restores antitumor immunity in LKB1 mutant lung cancer. Nat Cancer. 2021;2:503-514 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Morita K, Hama Y, Izume T, Tamura N, Ueno T, Yamashita Y, et al. Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formation. J Cell Biol. 2018;217:3817-3828 pubmed publisher
product information
Catalog Number :
117413
Product Name :
pMXs GFP-LC3-RFP
article :
doi10.1083/jcb.201804132
id28196555
pubmed_id30093494
bacterial resistance :
Ampicillin
cloning :
backbonepMXs-IP
backbone_mutation
backbone_originDr. Toshio Kitamura of the University of Tokyo
backbone_size5847
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.
origin :
37
pi :
alt_names
Map1lc3b
LC3
LC3B
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5CGACCACTACCAGCAGAACA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLC3B, Map1lc3, Mpl3, zbs559
geneMap1lc3b
id64862
genbank_ids
NM_022867.2
mutation
namemicrotubule-associated protein 1 light chain 3 beta
shRNA_sequence
size2200
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagEGFP
locationC terminal on insert
tagmRFP1
plasmid copy :
It has the pMX backbone
resistance markers :
644
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA