This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'
catalog :
117323
citations: 1
Reference
Kutsche L, Gysi D, Fallmann J, Lenk K, Petri R, Swiersy A, et al. Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Cell Syst. 2018;7:438-452.e8 pubmed publisher
product information
Catalog Number :
117323
Product Name :
pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'
article :
doi10.1016/j.cels.2018.08.011
id28196606
pubmed_id30292704
bacterial resistance :
Ampicillin
cloning :
backbonePX459
backbone_mutation
backbone_originZhang Lab
backbone_size9157
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cas9 with NLS included
growth strain :
CRISPR-Cas9 for miR-124-3, 5'
origin :
37
pi :
alt_names
NR_029670
ENSG00000207598
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5BbsI
promoterU6
sequencing_primer_3
sequencing_primer_5TTTCTTGGGTAGTTTGCAGTTTT
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesMIRN124-3, MIRN124A3, mir-124-3
geneMIR124-3
id406909
genbank_ids
mutation
namegRNA miR-124-3-5'
shRNA_sequencegtctatacaattaaggcacg
size20
species
100
Synthetic
tags
locationN terminal on backbone
tag3xFLAG
resistance markers :
3728
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA