This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PB09-TRE-beta-globin-miR-124-EF1a-GFP
catalog :
117318
citations: 1
Reference
Kutsche L, Gysi D, Fallmann J, Lenk K, Petri R, Swiersy A, et al. Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Cell Syst. 2018;7:438-452.e8 pubmed publisher
product information
Catalog Number :
117318
Product Name :
PB09-TRE-beta-globin-miR-124-EF1a-GFP
article :
doi10.1016/j.cels.2018.08.011
id28196606
pubmed_id30292704
bacterial resistance :
Ampicillin
cloning :
backbonePB-TRE
backbone_mutation
backbone_originChurch Lab
backbone_size7460
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Transposon-mediated integration
growth notes :
5' BamHI cloning site not destroyed, 3' BamHI cloning site destroyed
growth strain :
Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron
origin :
37
pi :
sapienstags
locationC terminal on backbone
tagGFP

mir-124-1gene>MIR124-1id4
06907e>genbank_ids>mutationd>namehsa-miR-124
-1shRNA_sequence
aggcctctctctccgtgttcacagcggaccttgat
ttaaatgtccatacaattaaggcacgcggtgaatgccaa
gaatggggctgsize<
td>2560speciesd>
9606d>
Homo
alt_names
NR_029668
ENST00000385275.1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTet-inducible promoter
sequencing_primer_3aagtcgtgctgcttcatgtg
sequencing_primer_5CCGTACCACTTCCTACCCTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIR124A, MIR124A1, MIRN124-1, MIRN124A1,
resistance markers :
3728
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA