This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MSCV-Scara3-IRES-BFP
catalog :
117265
citations: 1
Reference
Henriksson J, Chen X, Gomes T, Ullah U, Meyer K, Miragaia R, et al. Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. Cell. 2019;176:882-896.e18 pubmed publisher
product information
Catalog Number :
117265
Product Name :
MSCV-Scara3-IRES-BFP
article :
doi10.1016/j.cell.2018.11.044
id28192016
pubmed_id30639098
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV
backbone_mutation
backbone_origin
backbone_size
promoterLTR
sequencing_primer_3
sequencing_primer_5CTTGAACCTCCTCGTTCGAC
vector_types
Mammalian Expression
Retroviral
growth notes :
Addgene NGS results identified a G469C variant compared to the NCBI referense [NP_766192.1]. Plasmid should function as described in the associated publication.
growth strain :
Retroviral overexpression of Scara3
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterLTR
sequencing_primer_3
sequencing_primer_5CTTGAACCTCCTCGTTCGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPC7, C130058N24Rik, CSR, CSR1, MSLR1, MSRL1
geneScara3
id219151
genbank_ids
mutationSee depositor comments below.
nameScara3
shRNA_sequence
size
species
10090
Mus musculus
tags
resistance markers :
3639
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA