pEP PolI | Addgene 11722 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEP PolI

catalog :

11722

citations: 2

Reference
Otoupal P, Chatterjee A. CRISPR Gene Perturbations Provide Insights for Improving Bacterial Biofuel Tolerance. Front Bioeng Biotechnol. 2018;6:122 pubmed publisher
Camps M, Naukkarinen J, Johnson B, Loeb L. Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. Proc Natl Acad Sci U S A. 2003;100:9727-32 pubmed

product information

Catalog Number :

11722

Product Name :

pEP PolI

article :

doi	10.1073/pnas.1333928100
id	640
pubmed_id	12909725

bacterial resistance :

Chloramphenicol

cloning :

backbone

pHSG576

backbone_mutation

backbone_origin

see NCBI D88215

backbone_size

3475

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

See attached mutagenesis protocol provided by depositing scientist. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at help@addgene.org or contact our distributors if you have any questions.

growth temp :

Grow at 30oC in LB in JS200 cells (polA recA718). The cells need to be kept in exponential phase (do not let the culture reach saturation)!

inserts :

JS200

origin :

pi :

alt_names

DNA polymerase I

PolA

PolI

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	HindIII
promoter
sequencing_primer_3	m13 forward 20 primer
sequencing_primer_5	m13 reverse primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	b3863, ECK3855, JW3835, resA
gene	polA
id	948356

genbank_ids

mutation

D424A, I709N, A759R. Error-prone version of PolI.

name

DNA polymerase I EP

shRNA_sequence

size

2787

species

E. coli