This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pASK-mSAN1-Streptag
catalog :
117165
citations: 1
Reference
Andrews A, McCartney H, Errington T, D Andrea A, Macara I. A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. Nat Commun. 2018;9:2592 pubmed publisher
product information
Catalog Number :
117165
Product Name :
pASK-mSAN1-Streptag
article :
doi10.1038/s41467-018-05008-8 [pii]
id28196395
pubmed_id29968717
bacterial resistance :
Ampicillin
cloning :
backbonepASK-IBA3Plus
backbone_mutation
backbone_originIBA Lifesciences
backbone_size3172
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
MGC cDNA was a partial clone, repaired by the authors to generate full length mSAN1. mSAN1 contains a difference compared to NM_001357822.1 that results in a V549I protein-coding change.
growth strain :
Expresses Strep-tagged murine SAN1 nuclease in bacteria
growth temp :
Grow at 18oC after induction with tetracycline
origin :
37
pi :
alt_names
CCPG; fam120b
cloning
clone_methodRestriction Enzyme
cloning_site_3Bsa1
cloning_site_5Bsa1
promotertetR/tetA
sequencing_primer_3
sequencing_primer_5custom
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4932442K08Rik, AW049283, AW413338, CCPG, Pgcc1, mKIAA1838
geneFam120b
id67544
genbank_ids
NM_001357822.1
mutation
namemSAN1
shRNA_sequence
size2355
species
10090
Mus musculus
tags
locationC terminal on insert
tagStreptag
plasmid copy :
MGC cDNA library
resistance markers :
305
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA