This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVUHshp53-tTR-KRAB
catalog :
11654
citations: 2
Reference
Correia L, Johnson J, McErlean P, Bauer J, Farah H, Rassl D, et al. SOX2 Drives Bronchial Dysplasia in a Novel Organotypic Model of Early Human Squamous Lung Cancer. Am J Respir Crit Care Med. 2017;195:1494-1508 pubmed publisher
Szulc J, Wiznerowicz M, Sauvain M, Trono D, Aebischer P. A versatile tool for conditional gene expression and knockdown. Nat Methods. 2006;3:109-16 pubmed
product information
Catalog Number :
11654
Product Name :
pLVUHshp53-tTR-KRAB
article :
doi10.1038/nmeth846
id630
pubmed_id16432520
bacterial resistance :
Ampicillin
cloning :
backbonepLVU-tTR-KRAB
backbone_mutation
backbone_origin
backbone_size11579
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
Cre/Lox
growth notes :
Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
origin :
37
pi :
alt_names
shRNA against p53
p53
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5See map
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namehUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
6
532
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA