pLVUHshp53 | Addgene 11653 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVUHshp53

catalog :

11653

citations: 8

Reference
Rakhmatullina A, Mingaleeva R, Gafurbaeva D, Glazunova O, Sagdeeva A, Bulatov E, et al. Adipose-Derived Mesenchymal Stem Cell (MSC) Immortalization by Modulation of hTERT and TP53 Expression Levels. J Pers Med. 2023;13: pubmed publisher
Goldhammer N, Kim J, Villadsen R, Rønnov Jessen L, Petersen O. Myoepithelial progenitors as founder cells of hyperplastic human breast lesions upon PIK3CA transformation. Commun Biol. 2022;5:219 pubmed publisher
Molinuevo R, Freije A, Contreras L, Sanz J, Gandarillas A. The DNA damage response links human squamous proliferation with differentiation. J Cell Biol. 2020;219: pubmed publisher
Freije A, Sanz Gómez N, Gandarillas A. Genetic Modification of Human Primary Keratinocytes by Lentiviral Vectors. Methods Mol Biol. 2019;: pubmed publisher
de Pedro I, Alonso Lecue P, Sanz Gómez N, Freije A, Gandarillas A. Sublethal UV irradiation induces squamous differentiation via a p53-independent, DNA damage-mitosis checkpoint. Cell Death Dis. 2018;9:1094 pubmed publisher
Rodriguez R, Tornin J, Suarez C, Astudillo A, Rubio R, Yauk C, et al. Expression of FUS-CHOP fusion protein in immortalized/transformed human mesenchymal stem cells drives mixoid liposarcoma formation. Stem Cells. 2013;31:2061-72 pubmed publisher
Sandler V, Lailler N, Bouhassira E. Reprogramming of embryonic human fibroblasts into fetal hematopoietic progenitors by fusion with human fetal liver CD34+ cells. PLoS ONE. 2011;6:e18265 pubmed publisher
Szulc J, Wiznerowicz M, Sauvain M, Trono D, Aebischer P. A versatile tool for conditional gene expression and knockdown. Nat Methods. 2006;3:109-16 pubmed

product information

Catalog Number :

11653

Product Name :

pLVUHshp53

article :

doi	10.1038/nmeth846
id	630
pubmed_id	16432520

bacterial resistance :

Ampicillin

cloning :

backbone

None

backbone_mutation

backbone_origin

backbone_size

9931

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

Cre/Lox

growth notes :

Please visit the Trono lab website: http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols. shRNA sequence 5'-AGTAGATTACCACTGGAGTCTT

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

origin :

pi :

alt_names

shRNA against p53

p53

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	See map
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

name

GFP, shRNA against TP53

shRNA_sequence

size

species

9606

Homo sapiens