pLVPT-GDNF-rtTR-KRAB-2SM2 | Addgene 11647 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVPT-GDNF-rtTR-KRAB-2SM2

catalog :

11647

citations: 3

Reference
Alladin A, Chaible L, Garcia Del Valle L, Sabine R, Loeschinger M, Wachsmuth M, et al. Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation. elife. 2020;9: pubmed publisher
Shakhbazau A, Mohanty C, Shcharbin D, Bryszewska M, Caminade A, Majoral J, et al. Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve. J Control Release. 2013;172:841-51 pubmed publisher
Szulc J, Wiznerowicz M, Sauvain M, Trono D, Aebischer P. A versatile tool for conditional gene expression and knockdown. Nat Methods. 2006;3:109-16 pubmed

product information

Catalog Number :

11647

Product Name :

pLVPT-GDNF-rtTR-KRAB-2SM2

article :

doi	10.1038/nmeth846
id	630
pubmed_id	16432520

bacterial resistance :

Ampicillin

cloning :

backbone

pLVPT-rtTR-KRAB-2SM2

backbone_mutation

backbone_origin

backbone_size

11516

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

origin :

pi :

alt_names

GDNF

cloning

clone_method	Restriction Enzyme
cloning_site_3	See map
cloning_site_5	See map
promoter
sequencing_primer_3
sequencing_primer_5	See map
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATF, ATF1, ATF2, HFB1-GDNF, HSCR3
gene	GDNF
id	2668

genbank_ids

mutation

name

mPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off

shRNA_sequence

size

species

9606

Homo sapiens