pLVPT-GDNF-tTR-KRAB | Addgene 11646 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVPT-GDNF-tTR-KRAB

catalog :

11646

citations: 2

Reference
Shakhbazau A, Mohanty C, Shcharbin D, Bryszewska M, Caminade A, Majoral J, et al. Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve. J Control Release. 2013;172:841-51 pubmed publisher
Szulc J, Wiznerowicz M, Sauvain M, Trono D, Aebischer P. A versatile tool for conditional gene expression and knockdown. Nat Methods. 2006;3:109-16 pubmed

product information

Catalog Number :

11646

Product Name :

pLVPT-GDNF-tTR-KRAB

article :

doi	10.1038/nmeth846
id	630
pubmed_id	16432520

bacterial resistance :

Ampicillin

cloning :

backbone

pLVPT-tTR-KRAB

backbone_mutation

backbone_origin

backbone_size

11510

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

origin :

pi :

alt_names

GDNF

cloning

clone_method	Restriction Enzyme
cloning_site_3	See map
cloning_site_5	See map
promoter
sequencing_primer_3
sequencing_primer_5	See map
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATF, ATF1, ATF2, HFB1-GDNF, HSCR3
gene	GDNF
id	2668

genbank_ids

mutation

name

mPGK, GDNF, tTR-KRAB, Tet-on

shRNA_sequence

size

species

9606

Homo sapiens