pGEX2T merlin 1-595 | Addgene 11629 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX2T merlin 1-595

catalog :

11629

citations: 3

Reference
Primi M, Rangarajan E, Patil D, Izard T. Conformational flexibility determines the Nf2/merlin tumor suppressor functions. Matrix Biol Plus. 2021;12:100074 pubmed publisher
Chinthalapudi K, Mandati V, Zheng J, Sharff A, Bricogne G, Griffin P, et al. Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2. Nat Commun. 2018;9:1338 pubmed publisher
Gonzalez Agosti C, Wiederhold T, Herndon M, Gusella J, Ramesh V. Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF. J Biol Chem. 1999;274:34438-42 pubmed

product information

Catalog Number :

11629

Product Name :

pGEX2T merlin 1-595

article :

doi	10.1074/jbc.274.48.34438
id	627
pubmed_id	10567424

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX2T

backbone_mutation

backbone_origin

Amersham

backbone_size

4950

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The merlin insert in this plasmid contains A190V and G302E mutations compared to the closest match human Merlin reference sequence (NP_000259.1). The depositing laboratory states that these mutations should not affect merlin function, but cautions that it may be necessary to test merlin function if performing structure function experiments.

origin :

pi :

alt_names

NF2

isoform 1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	pGEX5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ACN, BANF, SCH, SWNV, merlin-1
gene	NF2
id	4771

genbank_ids

mutation

name

merlin

shRNA_sequence

size

1785

species

9606

Homo sapiens