This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3 proHB-EGF NC
catalog :
11605
citations: 1
Reference
Kim J, Adam R, Freeman M. Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress. Cancer Res. 2005;65:8242-9 pubmed
product information
Catalog Number :
11605
Product Name :
pcDNA3 proHB-EGF NC
article :
doi10.1158/0008-5472.CAN-05-0942
id622
pubmed_id16166300
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1-myc/His(A)
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
JM domain of HB-EGF replaced with JM domain of CD4 (PVENPLYTYDHT replaced with KVLPTWSTPVQP). The original citation is Suzuki et al., 1997. J Biol Chem 272(50), 31730-737.
origin :
37
pi :
alt_names
proHB-EGF
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDTR, DTS, DTSF, HEGFL
geneHBEGF
id1839
genbank_ids
mutationNon-cleavable HB-EGF: Pro149 to Thr160 replaced with CD4 JM domain.
nameHB-EGF
shRNA_sequence
size667
species
9606
Homo sapiens
tags
plasmid copy :
We received a proHB-EGF plasmid incorporating the CD4 JM domain from Michael Klagsbrun, Vascular Biology Research program, Children's Hospital Boston. The original citation is Suzuki et al., 1997. J Biol Chem 272(50), 31730-737.
resistance markers :
3
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA