This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLHCX-XBP1 mNeonGreen NLS
catalog :
115971
citations: 3
Reference
Chu H, Peterson C, Chamling X, Berlinicke C, Zack D, Jun A, et al. Integrated Stress Response Regulation of Corneal Epithelial Cell Motility and Cytokine Production. Invest Ophthalmol Vis Sci. 2022;63:1 pubmed publisher
Fuentes Iglesias A, Ameneiro C, Guallar D, Fidalgo M. Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells. Methods Mol Biol. 2021;: pubmed publisher
Nougarède A, Tesnière C, Ylanko J, Rimokh R, Gillet G, Andrews D. Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used for Image Segmentation. Assay Drug Dev Technol. 2018;16:350-360 pubmed publisher
product information
Catalog Number :
115971
Product Name :
pLHCX-XBP1 mNeonGreen NLS
article :
doi10.1089/adt.2018.862
id28196507
pubmed_id30088945
bacterial resistance :
Ampicillin
cloning :
backbonepLHCX
backbone_mutation
backbone_originClontech
backbone_size6813
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
This sensor leads to the expression of a mNeonGreen fusion protein in the nucleus upon induction of ER-stress. ER-stress kinetic through the IRE1/XBP1 pathway can thus be monitored by recording the green fluorescent signal arising in the nucleus. Designed by Adrien Nougar de.
growth strain :
ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3ACCTACAGGTGGGGTCTTTCATTCCC
sequencing_primer_5AGCTCGTTTAGTGAACCGTCAGATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTREB-5, TREB5, XBP-1, XBP2
geneXBP1
id7494
genbank_ids
mutation
nameX-box binding protein 1
shRNA_sequence
size1073
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagHA tag
locationC terminal on insert
tagmNeonGreen
locationC terminal on insert
tagc-myc NLS
resistance markers :
4132
tags :
Low Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA