This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVX-ATF4 mScarlet NLS
catalog :
115969
citations: 4
Reference
Fang Z, Corbizi Fattori G, McKerrell T, Boucher R, Jackson A, Fletcher R, et al. Tamoxifen for the treatment of myeloproliferative neoplasms: A Phase II clinical trial and exploratory analysis. Nat Commun. 2023;14:7725 pubmed publisher
Wang H, Pan X, Xiang X, Zhang Y, Chen J, Wen S, et al. CRISPR screen identifies the role of RBBP8 in mediating unfolded protein response induced liver damage through regulating protein synthesis. Cell Death Dis. 2023;14:531 pubmed publisher
Li M, Teater M, Hong J, Park N, Duy C, Shen H, et al. Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival. Blood Cancer Discov. 2022;3:50-65 pubmed publisher
Nougarède A, Tesnière C, Ylanko J, Rimokh R, Gillet G, Andrews D. Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used for Image Segmentation. Assay Drug Dev Technol. 2018;16:350-360 pubmed publisher
product information
Catalog Number :
115969
Product Name :
pLVX-ATF4 mScarlet NLS
article :
doi10.1089/adt.2018.862
id28196507
pubmed_id30088945
bacterial resistance :
Ampicillin
cloning :
backbonepLVX-Puro
backbone_mutation
backbone_originClontech
backbone_size8102
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
This sensor leads to the expression of a mScarlet-I fusion protein in the nucleus upon induction of ER-stress. ER-stress kinetic through the PERK/ATF4 pathway can thus be monitored by recording the red fluorescent signal arising in the nucleus. Designed by Adrien Nougar de.
growth strain :
ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3CAGCGGGGCTGCTAAAGCGCATGC
sequencing_primer_5AGCTCGTTTAGTGAACCGTCAGATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCREB-2, CREB2, TAXREB67, TXREB
geneATF4
id468
genbank_ids
mutation
nameactivating transcription factor 4
shRNA_sequence
size1166
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagHA tag
locationC terminal on insert
tagmScarlet-I
locationC terminal on insert
tagc-myc NLS
resistance markers :
4132
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA