pLVX-XBP1 mNeonGreen NLS | Addgene 115968 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVX-XBP1 mNeonGreen NLS

catalog :

115968

citations: 1

Reference
Nougarède A, Tesnière C, Ylanko J, Rimokh R, Gillet G, Andrews D. Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used for Image Segmentation. Assay Drug Dev Technol. 2018;16:350-360 pubmed publisher

product information

Catalog Number :

115968

Product Name :

pLVX-XBP1 mNeonGreen NLS

article :

doi	10.1089/adt.2018.862
id	28196507
pubmed_id	30088945

bacterial resistance :

Ampicillin

cloning :

backbone

pLVX-Puro

backbone_mutation

backbone_origin

Clontech

backbone_size

8102

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

This sensor leads to the expression of a mNeonGreen fusion protein in the nucleus upon induction of ER-stress. ER-stress kinetic through the IRE1/XBP1 pathway can thus be monitored by recording the green fluorescent signal arising in the nucleus. Designed by Adrien Nougar de.

growth strain :

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	CAGCGGGGCTGCTAAAGCGCATGC
sequencing_primer_5	AGCTCGTTTAGTGAACCGTCAGATC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	TREB-5, TREB5, XBP-1, XBP2
gene	XBP1
id	7494

genbank_ids

mutation

name

X-box binding protein 1

shRNA_sequence

size

1061

species

9606

Homo sapiens

100

Synthetic