This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA 3.3 hFZD4
catalog :
115908
citations: 1
Reference
Lai M, Zhang C, Shi J, Johnson V, Khandan L, McVey J, et al. TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling. Cell Rep. 2017;19:2809-2822 pubmed publisher
product information
Catalog Number :
115908
Product Name :
pcDNA 3.3 hFZD4
article :
doi10.1016/j.celrep.2017.06.004
id28191514
pubmed_id28658627
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3 TOPO TA
backbone_mutation
backbone_origin
backbone_size5407
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
TOPFlash reporter assay can be inhibited by transfection of too much of this plasmid (see Lai et al., 2017 for detailed description of transfection conditions).
growth strain :
Expresses human FZD4 in mammalian cells.
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CTTCCGTGTTTCAGTTAGC
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD344, EVR1, FEVR, FZD4S, Fz-4, Fz4, FzE4, GPCR, hFz4
geneFZD4
id8322
genbank_ids
mutation
nameFZD4
shRNA_sequence
size1619
species
9606
Homo sapiens
tags
plasmid copy :
Image clone 5199771
resistance markers :
4135
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA