pcDNA 3.3 ss-V5-hFZD4 (aa 37-537) | Addgene 115787 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

catalog :

115787

citations: 1

Reference
Lai M, Zhang C, Shi J, Johnson V, Khandan L, McVey J, et al. TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling. Cell Rep. 2017;19:2809-2822 pubmed publisher

product information

Catalog Number :

115787

Product Name :

pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

article :

doi	10.1016/j.celrep.2017.06.004
id	28191514
pubmed_id	28658627

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.3 TOPO TA

backbone_mutation

backbone_origin

backbone_size

5407

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

N-terminal V5 tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control. TOPFlash reporter assay can be inhibited by transfection of too much of this plasmid (see Lai et al., 2017 for detailed description of transfection conditions).

growth strain :

Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	AgeI
cloning_site_5	XbaI
promoter	CMV
sequencing_primer_3	CTTCCGTGTTTCAGTTAGC
sequencing_primer_5	CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD344, EVR1, FEVR, FZD4S, Fz-4, Fz4, FzE4, GPCR, hFz4
gene	FZD4
id	8322

genbank_ids

mutation

name

FZD4

shRNA_sequence

size

1640

species

9606

Homo sapiens