This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA 3.3 HA-hTSPAN12
catalog :
115784
citations: 1
Reference
Lai M, Zhang C, Shi J, Johnson V, Khandan L, McVey J, et al. TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand Selectivity and Signaling. Cell Rep. 2017;19:2809-2822 pubmed publisher
product information
Catalog Number :
115784
Product Name :
pcDNA 3.3 HA-hTSPAN12
article :
doi10.1016/j.celrep.2017.06.004
id28191514
pubmed_id28658627
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3 TOPO TA
backbone_mutation
backbone_originInvitrogen
backbone_size5407
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
N-terminal HA tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control
growth strain :
Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CTTCCGTGTTTCAGTTAGC
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEVR5, NET-2, NET2, TM4SF12
geneTSPAN12
id23554
genbank_ids
mutationmultiple silent mutations introduce restriction sites to enable chimera construction
nameTSPAN12
shRNA_sequence
size970
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
plasmid copy :
gene synthesis
resistance markers :
4135
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA