This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLTR.gp140/EGFP.Revdelta38/DsRed
catalog :
115775
citations: 1
Reference
Khoury G, Purcell D. High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing. J Vis Exp. 2019;: pubmed publisher
product information
Catalog Number :
115775
Product Name :
pLTR.gp140/EGFP.Revdelta38/DsRed
article :
doi10.3791/58753
id28196488
pubmed_id30735158
bacterial resistance :
Ampicillin
cloning :
backbonepUC18
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Also see Khoury et al. Retrovirology (2018) 15:36 https://doi.org/10.1186/s12977-018-0421-6
growth strain :
HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterHIV-1 (LTR)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationgp140 uncleaved (1190-91aa, KR > TG; truncated prior to the transmembrane domain at amino acid 1363); Revdelta38
nameEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
shRNA_sequence
size
species
32644
Other
tags
locationC terminal on insert
tagEGFP; dsReD
resistance markers :
4124
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA