pAav-TP53-T2A-BirA* | Addgene 115652 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAav-TP53-T2A-BirA*

catalog :

115652

citations: 1

Reference
Vandemoortele G, De Sutter D, Moliere A, Pauwels J, Gevaert K, Eyckerman S. A Well-Controlled BioID Design for Endogenous Bait Proteins. J Proteome Res. 2019;18:95-106 pubmed publisher

product information

Catalog Number :

115652

Product Name :

pAav-TP53-T2A-BirA*

article :

doi	10.1101/427807
id	28196461
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pAav

backbone_mutation

backbone_origin

Agilent technologies

backbone_size

2907

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth strain :

rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

origin :

pi :

BMFS5, LFS1, P53, TRP53geneTP53id7157genbank_idsmutationHomology region 1nameTP53shRNA_sequencesize883species

9606

Homo sapiens

tags

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NheI
cloning_site_5	KpnI
promoter
sequencing_primer_3	GGGTTCCTGCGGCCGCTTTTGCTAGCAGATCACGCCACTCCACTCC
sequencing_primer_5	GGGAGGATTGGGAAGACAAT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

Homology region 2

name

TP53

shRNA_sequence

size

838

species

9606

Homo sapiens

tags

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	TAGGGCGCGATAACTTCGTA
sequencing_primer_5	CACTAGGGGTTCCTGCGGCCGCAAACGCGTCAGCCCTGCACAGACATTTT
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

T2A

shRNA_sequence

size

species

100

Synthetic

tags

location
tag	T2A

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	NdeI
promoter
sequencing_primer_3	TAGGGCGCGATAACTTCGTA
sequencing_primer_5	ACATATTTGCATGGGGTGTG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

BirA*

shRNA_sequence

size

species

32644

Other

E. coli

tags

location
tag	BirA*

Enzymecloning_site_3<
/td>NdeIcloning_s
ite_5MluIpro
motersequenc
ing_primer_3TAGGGCGCGATAACTTCG
TAsequencing_primer_5
CACTAGGGGTTCCTGCGGCCGCAAACGCGT
CAGCCCTGCACAGACATTTTs
ite_3_destroyed<
td>site_5_destroyed<
/table>entrez_gened>

ali
ases

BCC7,

alt_names

cloning

clone_method

Restriction

resistance markers :

2951

tags :

High Copy

terms :

Neomycin (select with G418)