pNJP | Addgene 115494 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pNJP

catalog :

115494

citations: 3

Reference
Gr xf6 nloh M, Arts J, Mahlandt E, Nolte M, Goedhart J, van Buul J. Primary adhered neutrophils increase JNK1-MARCKSL1-mediated filopodia to promote secondary neutrophil transmigration. iScience. 2023;26:107406 pubmed publisher
Kamada H, Yasuhira S, Shibazaki M, Amano H, Maesawa C. DUSP4 Inactivation Leads to Reduced Extracellular Signal‒Regulated Kinase Activity through Upregulation of DUSP6 in Melanoma Cells. J Invest Dermatol. 2022;: pubmed publisher
Miura H, Kondo Y, Matsuda M, Aoki K. Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK Causes Stochastic Cell Death. Cell Rep. 2018;24:2658-2668 pubmed publisher

product information

Catalog Number :

115494

Product Name :

pNJP

article :

doi	10.1016/j.celrep.2018.08.020
id	28196416
pubmed_id	30184500

bacterial resistance :

Ampicillin

cloning :

backbone

pPB

backbone_mutation

backbone_origin

Allan Bradley (Wellcome Sanger Institute)

backbone_size

6804

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

For JNK KTR, see also: Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.

growth strain :

Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

origin :

pi :

alt_names

NJP (Nuclear, JNK, and p38 reporter)

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CAG
sequencing_primer_3	gggccctcacattgccaaa
sequencing_primer_5	catgttcatgccttcttctttttcc
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA
gene	MAPK14
id	1432

aliases	JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
gene	MAPK8
id	5599

genbank_ids

mutation

mCherry S227F, mCherry G229R, mKO V211G

name

NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2

shRNA_sequence

size

3918

species

9606

Homo sapiens

10090

Mus musculus

100

Synthetic