This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLIC.B3.TM1734
catalog :
11527
citations: 1
Reference
Liu J, Lou Y, Yokota H, Adams P, Kim R, Kim S. Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters. J Biol Chem. 2005;280:15960-6 pubmed publisher
product information
Catalog Number :
11527
Product Name :
pLIC.B3.TM1734
article :
doi10.1074/jbc.M414117200
id586
pubmed_id15716271
bacterial resistance :
Ampicillin
cloning :
backbonepLIC.B3
backbone_mutation
backbone_origin
backbone_size6194
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid pLIC.B3.TM1734 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SmaI
promoter
sequencing_primer_3GTTGCATCACCTTCACCCTCTCC
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesTM1734
geneTM1734
id897173
genbank_ids
AAD36799
TM1734
NP_229532
mutation
namePhoU
shRNA_sequence
size705
species
T. maritima
tags
locationN terminal on backbone
tagHisTag, TEV cleavable
resistance markers :
251
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA