This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-UbC-mCherry Rab5
catalog :
115239
citations: 1
Reference
Wang Y, Leung V, Zhang Y, Nudell V, Loud M, Servin Vences M, et al. The role of somatosensory innervation of adipose tissues. Nature. 2022;609:569-574 pubmed publisher
product information
Catalog Number :
115239
Product Name :
pAAV-UbC-mCherry Rab5
article :
doi
id28196369
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-SIBR-anti_I
backbone_mutationThe anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
backbone_originChung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
backbone_size5235
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth notes :
Note: Plasmid contains a mutation that removes the first nine amino acids of mCherry. This mutation is not known to affect plasmid function.
growth strain :
To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
origin :
37
pi :
alt_names
NP_004153.2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoterHuman UbC
sequencing_primer_3
sequencing_primer_5pCAG_F_primer
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesRAB5
geneRAB5A
id5868
genbank_ids
NP_004153.2
mutation
nameRab5a
shRNA_sequence
size1340
species
9606
Homo sapiens
32644
Other
Homo sapiens
tags
locationN terminal on insert
tagmCherry
resistance markers :
1214
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA