This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-UbC-mEmerald Rab7a
catalog :
115238
product information
Catalog Number :
115238
Product Name :
pAAV-UbC-mEmerald Rab7a
article :
doi
id28196369
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepAAV-SIBR-anti_I
backbone_mutationThe anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
backbone_originChung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
backbone_size5235
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth strain :
To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.
origin :
37
pi :
alt_names
NM_004637.5
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5BamHI
promoterHuman UbC
sequencing_primer_3
sequencing_primer_5pCAG_F_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMT2B, PRO2706, RAB7
geneRAB7A
id7879
genbank_ids
NM_004637.5
mutation7 Amino Acids (21 Nucleotides) between mEmerald and Rab7a
nameRab7a fused to mEmerald
shRNA_sequence
size1416
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmEmerald
locationN terminal on insert
tagmEmerald
plasmid copy :
ADDGENE plasmid #54244
resistance markers :
1214
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA