This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLEX307_CR-PDHA2S293A
catalog :
115204
citations: 1
Reference
Moutaoufik M, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, et al. Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. iScience. 2019;19:1114-1132 pubmed publisher
product information
Catalog Number :
115204
Product Name :
pLEX307_CR-PDHA2S293A
article :
doi10.1016/j.isci.2019.08.057
id28203664
pubmed_id31536960
bacterial resistance :
Ampicillin
cloning :
backbonepLEX-307
backbone_mutation
backbone_originDavid Root, Addgene plasmid # 41392
backbone_size8270
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5NheI
promoterEF1alpha
sequencing_primer_3WPRE_R
sequencing_primer_5EF1alpha_F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPDHAL, MGC149517, MGC149518
genePDHA2
id5161
genbank_ids
NM_005390.4, NP_005381.1
mutationc.321C>T (silent when translated to protein, confers CRISPR/Cas9 resistance since it mutates the canonical PAM site), p.S293A
nameCR (CRISPR/Cas9-resistant)-PDHA2S293A
shRNA_sequence
size1167
species
9606
Homo sapiens
tags
resistance markers :
3055
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA