pLEX307_CR-PDHA2S291D/S293D | Addgene 115202 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLEX307_CR-PDHA2S291D/S293D

catalog :

115202

citations: 1

Reference
Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik M, Zilocchi M, et al. The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF. Nucleic Acids Res. 2021;: pubmed publisher

product information

Catalog Number :

115202

Product Name :

pLEX307_CR-PDHA2S291D/S293D

article :

doi	10.1093/nar/gkab585
id	28216408
pubmed_id	34244791

bacterial resistance :

Ampicillin

cloning :

backbone

pLEX-307

backbone_mutation

backbone_origin

David Root, Addgene plasmid # 41392

backbone_size

8270

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth strain :

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	NheI
promoter	EF1alpha
sequencing_primer_3	WPRE_R
sequencing_primer_5	EF1alpha_F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PDHAL, MGC149517, MGC149518
gene	PDHA2
id	5161

genbank_ids

NM_005390.4, NP_005381.1

mutation

c.321C>T (silent when translated to protein, confers CRISPR/Cas9 resistance since it mutates the canonical PAM site), p.S291D/S293D

name

CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D

shRNA_sequence

size

1167

species

9606

Homo sapiens