This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDest-565
catalog :
11520
citations: 5
Reference
Khan O, Almagro J, Nelson J, Horswell S, Encheva V, Keyan K, et al. Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12. Nat Commun. 2021;12:2043 pubmed publisher
Maeoka Y, Wu Y, Okamoto T, Kanemoto S, Guo X, Saito A, et al. NFAT5 up-regulates expression of the kidney-specific ubiquitin ligase gene Rnf183 under hypertonic conditions in inner-medullary collecting duct cells. J Biol Chem. 2019;294:101-115 pubmed publisher
McNeilly D, Schofield A, Stone S. Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system. Plant Mol Biol. 2018;96:265-278 pubmed publisher
Kaczmarczyk A, Vorholt J, Francez Charlot A. Synthetic vanillate-regulated promoter for graded gene expression in Sphingomonas. Sci Rep. 2014;4:6453 pubmed publisher
Kaczmarczyk A, Vorholt J, Francez Charlot A. Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013;79:6795-802 pubmed publisher
product information
Catalog Number :
11520
Product Name :
pDest-565
article :
doi
id580
pubmed_id
bacterial resistance :
Chloramphenicol and Ampicillin
cloning :
backbonepDest-560
backbone_mutation
backbone_originPEL
backbone_size8932
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
Gateway Destination Vector
growth strain :
Gateway destination vector for bacterial expression; His,GST tag
growth temp :
Requires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name
shRNA_sequence
size
species
tags
locationN terminal on backbone
tagHis6-GST
plasmid copy :
GST gene from Promega pGEX vector
resistance markers :
139
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA