pLD-puro-Cc-CR-PARK7C53A-VA | Addgene 115189 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLD-puro-Cc-CR-PARK7C53A-VA

catalog :

115189

citations: 1

Reference
Moutaoufik M, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, et al. Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. iScience. 2019;19:1114-1132 pubmed publisher

product information

Catalog Number :

115189

Product Name :

pLD-puro-Cc-CR-PARK7C53A-VA

article :

doi	10.1016/j.isci.2019.08.057
id	28203664
pubmed_id	31536960

bacterial resistance :

Ampicillin

cloning :

backbone

pLD-Cc-puro-VA

backbone_mutation

backbone_origin

Jason Moffat, Addgene plasmid # 24588

backbone_size

7737

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth strain :

Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	hPGK_R [5'-ccttggaaaaggcgcaacc-3']
sequencing_primer_5	CMV_F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DJ-1, DJ1, GATD2, HEL-S-67p
gene	PARK7
id	11315

genbank_ids

mutation

c.150G>A (silent when translated to protein, confers CRISPR/Cas9 resistance since it mutates the canonical PAM site), p.C53A

name

CR (CRISPR/Cas9-resistant)-PARK7C53A

shRNA_sequence

size

567

species

9606

Homo sapiens