pET32a-HD46Q | Addgene 11515 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET32a-HD46Q

catalog :

11515

citations: 4

Reference
Haver H, Wedemeyer M, Butcher E, Peterson F, Volkman B, Scaglione K. Mechanistic Insight into the Suppression of Polyglutamine Aggregation by SRCP1. ACS Chem Biol. 2023;18:549-560 pubmed publisher
Denis H, Alpaugh M, Alvarez C, Fenyi A, Barker R, Chouinard S, et al. Detection of antibodies against the huntingtin protein in human plasma. Cell Mol Life Sci. 2023;80:45 pubmed publisher
Belwal V, Datta D, Chaudhary N. The β-turn-supporting motif in the polyglutamine binding peptide QBP1 is essential for inhibiting huntingtin aggregation. FEBS Lett. 2020;: pubmed publisher
Bennett M, Huey Tubman K, Herr A, West A, Ross S, Bjorkman P. A linear lattice model for polyglutamine in CAG-expansion diseases. Proc Natl Acad Sci U S A. 2002;99:11634-9 pubmed

product information

Catalog Number :

11515

Product Name :

pET32a-HD46Q

article :

doi	10.1073/pnas.182393899
id	573
pubmed_id	12193654

bacterial resistance :

Ampicillin

cloning :

backbone

pET-32a

backbone_mutation

backbone_origin

Novagen

backbone_size

5900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

origin :

pi :

alt_names

huntingtin (Huntington disease)

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NcoI
promoter
sequencing_primer_3	T7 terminator
sequencing_primer_5	T7 promoter
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HD, IT15, LOMARS
gene	HTT
id	3064

genbank_ids

NM_002111

mutation

huntingtin exon 1 with 46 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.

name

huntingtin, exon 1, 46Q glutamines

shRNA_sequence

size

364

species

9606

Homo sapiens