pET32a-HD39Q | Addgene 11514 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET32a-HD39Q

catalog :

11514

citations: 2

Reference
Aviolat H, Pinto R, Godschall E, Murtha R, Richey H, Sapp E, et al. Assessing average somatic CAG repeat instability at the protein level. Sci Rep. 2019;9:19152 pubmed publisher
Bennett M, Huey Tubman K, Herr A, West A, Ross S, Bjorkman P. A linear lattice model for polyglutamine in CAG-expansion diseases. Proc Natl Acad Sci U S A. 2002;99:11634-9 pubmed

product information

Catalog Number :

11514

Product Name :

pET32a-HD39Q

article :

doi	10.1073/pnas.182393899
id	573
pubmed_id	12193654

bacterial resistance :

Ampicillin

cloning :

backbone

pET-32a

backbone_mutation

backbone_origin

Novagen

backbone_size

5900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

origin :

pi :

alt_names

huntingtin (Huntington disease)

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NcoI
promoter
sequencing_primer_3	T7 terminator
sequencing_primer_5	T7 promoter
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HD, IT15, LOMARS
gene	HTT
id	3064

genbank_ids

NM_002111

mutation

huntingtin exon 1 with 39 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.

name

huntingtin, exon 1, 39 glutamines

shRNA_sequence

size

351

species

9606

Homo sapiens