This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GSO2/EDA23 X079_pECIA2
catalog :
115111
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
115111
Product Name :
GSO2/EDA23 X079_pECIA2
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA2
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47032)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
EEDVLRDWNSGSPSYCNWTGVTCGGREIIGLNLSGLGLT
GSISPSIGRFNNLIHIDLSSNRLVGPIPTTLSNLSSSLE
SLHLFSNLLSGDIPSQLGSLVNLKSLKLGDNELNGTIPE
TFGNLVNLQMLALASCRLTGLIPSRFGRLVQLQTLILQD
NELEGPIPAEIGNCTSLALFAAAFNRLNGSLPAELNRLK
NLQTLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQGL
IPKRLTELANLQTLDLSSNNLTGVIHEEFWRMNQLEFLV
LAKNRLSGSLPKTICSNNTSLKQLFLSETQLSGEIPAEI
SNCQSLKLLDLSNNTLTGQIPDSLFQLVELTNLYLNNNS
LEGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKL
EIMYLYENRFSGEMPVEIGNCTRLQEIDWYGNRLSGEIP
SSIGRLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLA
DNQLSGSIPSSFGFLTALELFMIYNNSLQGNLPDSLINL
KNLTRINFSSNKFNGSISPLCGSSSYLSFDVTENGFEGD
IPLELGKSTNLDRLRLGKNQFTGRIPRTFGKISELSLLD
ISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGVIPTWLG
KLPLLGELKLSSNKFVGSLPTEIFSLTNILTLFLDGNSL
NGSIPQEIGNLQALNALNLEENQLSGPLPSTIGKLSKLF
ELRLSRNALTGEIPVEIGQLQDLQSALDLSYNNFTGRIP
STISTLPKLESLDLSHNQLVGEVPGQIGDMKSLGYLNLS
YNNLEGKLKKQFSRWQADAFVGNAGLCGSPLSHCNRAGS
KNQRSLS
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence:
growth strain :
Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT5G44700, EDA23, EMBRYO SAC DEVELOPMENT ARREST 23, GASSHO 2, K23L20.3, K23L20_3
geneGSO2
id834499
genbank_ids
mutation
nameAT5G44700
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagFc, V5 and hexahistidine tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA