This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AT1G35710 X075_pECIA2
catalog :
115098
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
115098
Product Name :
AT1G35710 X075_pECIA2
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA2
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47032)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
SATIAEANALLKWKSTFTNSSKLSSWVHDANTNTSFSCT
SWYGVSCNSRGSIEELNLTNTGIEGTFQDFPFISLSNLA
YVDLSMNLLSGTIPPQFGNLSKLIYFDLSTNHLTGEISP
SLGNLKNLTVLYLHQNYLTSVIPSELGNMESMTDLALSQ
NKLTGSIPSSLGNLKNLMVLYLYENYLTGVIPPELGNME
SMTDLALSQNKLTGSIPSTLGNLKNLMVLYLYENYLTGV
IPPEIGNMESMTNLALSQNKLTGSIPSSLGNLKNLTLLS
LFQNYLTGGIPPKLGNIESMIDLELSNNKLTGSIPSSLG
NLKNLTILYLYENYLTGVIPPELGNMESMIDLQLNNNKL
TGSIPSSFGNLKNLTYLYLYLNYLTGVIPQELGNMESMI
NLDLSQNKLTGSVPDSFGNFTKLESLYLRVNHLSGAIPP
GVANSSHLTTLILDTNNFTGFFPETVCKGRKLQNISLDY
NHLEGPIPKSLRDCKSLIRARFLGNKFTGDIFEAFGIYP
DLNFIDFSHNKFHGEISSNWEKSPKLGALIMSNNNITGA
IPTEIWNMTQLVELDLSTNNLFGELPEAIGNLTNLSRLR
LNGNQLSGRVPAGLSFLTNLESLDLSSNNFSSEIPQTFD
SFLKLHDMNLSRNKFDGSIPRLSKLTQLTQLDLSHNQLD
GEIPSQLSSLQSLDKLDLSHNNLSGLIPTTFEGMIALTN
VDISNNKLEGPLPDTPTFRKATADALEENIGLCSNIPKQ
RLKPCRELKKPKK
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence:
growth strain :
Bait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G35710, F14D7.1, F14D7_1
geneAT1G35710
id840475
genbank_ids
mutation
nameAT1G35710
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagFc, V5 and hexahistidine tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA