This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
BRI1 BRI1_pECIA2
catalog :
115086
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
115086
Product Name :
BRI1 BRI1_pECIA2
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA2
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47032)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
MVSAIVLYVLLAAAAHSAFAAMGSQSLYREIHQLISFKD
VLPDKNLLPDWSSNKNPCTFDGVTCRDDKVTSIDLSSKP
LNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSGFKCSA
SLTSLDLSRNSLSGPVTTLTSLGSCSGLKFLNVSSNTLD
FPGKVSGGLKLNSLEVLDLSANSISGANVVGWVLSDGCG
ELKHLAISGNKISGDVDVSRCVNLEFLDVSSNNFSTGIP
FLGDCSALQHLDISGNKLSGDFSRAISTCTELKLLNISS
NQFVGPIPPLPLKSLQYLSLAENKFTGEIPDFLSGACDT
LTGLDLSGNHFYGAVPPFFGSCSLLESLALSSNNFSGEL
PMDTLLKMRGLKVLDLSFNEFSGELPESLTNLSASLLTL
DLSSNNFSGPILPNLCQNPKNTLQELYLQNNGFTGKIPP
TLSNCSELVSLHLSFNYLSGTIPSSLGSLSKLRDLKLWL
NMLEGEIPQELMYVKTLETLILDFNDLTGEIPSGLSNCT
NLNWISLSNNRLTGEIPKWIGRLENLAILKLSNNSFSGN
IPAELGDCRSLIWLDLNTNLFNGTIPAAMFKQSGKIAAN
FIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRL
STRNPCNITSRVYGGHTSPTFDNNGSMMFLDMSYNMLSG
YIPKEIGSMPYLFILNLGHNDISGSIPDEVGDLRGLNIL
DLSSNKLDGRIPQAMSALTMLTEIDLSNNNLSGPIPEMG
QFETFPPAKFLNNPGLCGYPLPRCDPSNADGYAHHQRSH
GRR
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence:
growth strain :
Bait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G39400, ATBRI1, BIN1, BR INSENSITIVE 1, BRASSINOSTEROID INSENSITIVE 1, CABBAGE 2, CBB2, DWARF 2, DWF2, F23K16.30, F23K16_30
geneBRI1
id830095
genbank_ids
mutation
nameAT4G39400
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagFc, V5 and hexahistidine tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA