This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PSY1R C3_pECIA2
catalog :
115079
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
115079
Product Name :
PSY1R C3_pECIA2
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA2
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47032)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
LQDRDSLLWFSGNVSSPVSPLHWNSSIDCCSWEGISCDK
SPENRVTSIILSSRGLSGNLPSSVLDLQRLSRLDLSHNR
LSGPLPPGFLSALDQLLVLDLSYNSFKGELPLQQSFGNG
SNGIFPIQTVDLSSNLLEGEILSSSVFLQGAFNLTSFNV
SNNSFTGSIPSFMCTASPQLTKLDFSYNDFSGDLSQELS
RCSRLSVLRAGFNNLSGEIPKEIYNLPELEQLFLPVNRL
SGKIDNGITRLTKLTLLELYSNHIEGEIPKDIGKLSKLS
SLQLHVNNLMGSIPVSLANCTKLVKLNLRVNQLGGTLSA
IDFSRFQSLSILDLGNNSFTGEFPSTVYSCKMMTAMRFA
GNKLTGQISPQVLELESLSFFTFSDNKMTNLTGALSILQ
GCKKLSTLIMAKNFYDETVPSNKDFLRSDGFPSLQIFGI
GACRLTGEIPAWLIKLQRVEVMDLSMNRFVGTIPGWLGT
LPDLFYLDLSDNFLTGELPKELFQLRALMSQKAYDATER
NYLELPVFVNPNNVTTNQQYNQLSSLPPTIYIKRNNLTG
TIPVEVGQLKVLHILELLGNNFSGSIPDELSNLTNLERL
DLSNNNLSGRIPWSLTGLHFLSYFNVANNTLSGPIPTGT
QFDTFPKANFEGNPLLCGGVLLTSCDPTQHSTTKMGK
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence:
growth strain :
Bait vector PSY1R C3_pECIA2 should be used with prey vector PSY1R C3_pECIA14.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G72300, PSY1 receptor, T9N14.20, T9N14_20
genePSY1R
id843562
genbank_ids
mutation
nameAT1G72300
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagFc, V5 and hexahistidine tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA