This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EFR D5_pECIA14
catalog :
114919
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
114919
Product Name :
EFR D5_pECIA14
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA14
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47051)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
SNETDMQALLEFKSQVSENNKREVLASWNHSSPFCNWIG
VTCGRRRERVISLNLGGFKLTGVISPSIGNLSFLRLLNL
ADNSFGSTIPQKVGRLFRLQYLNMSYNLLEGRIPSSLSN
CSRLSTVDLSSNHLGHGVPSELGSLSKLAILDLSKNNLT
GNFPASLGNLTSLQKLDFAYNQMRGEIPDEVARLTQMVF
FQIALNSFSGGFPPALYNISSLESLSLADNSFSGNLRAD
FGYLLPNLRRLLLGTNQFTGAIPKTLANISSLERFDISS
NYLSGSIPLSFGKLRNLWWLGIRNNSLGNNSSSGLEFIG
AVANCTQLEYLDVGYNRLGGELPASIANLSTTLTSLFLG
QNLISGTIPHDIGNLVSLQELSLETNMLSGELPVSFGKL
LNLQVVDLYSNAISGEIPSYFGNMTRLQKLHLNSNSFHG
RIPQSLGRCRYLLDLWMDTNRLNGTIPQEILQIPSLAYI
DLSNNFLTGHFPEEVGKLELLVGLGASYNKLSGKMPQAI
GGCLSMEFLFMQGNSFDGAIPDISRLVSLKNVDFSNNNL
SGRIPRYLASLPSLRNLNLSMNKFEGRVPTTGVFRNATA
VSVFGNTNICGGVREMQLKPCIVQASPRKRK
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence:
growth strain :
Prey vector EFR D5_pECIA14 should be used with bait vector EFR D5_pECIA2.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT5G20480, EF-TU receptor, F7C8.70, F7C8_70
geneEFR
id832170
genbank_ids
mutation
nameAT5G20480
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA