This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
BARK1 X073_pECIA14
catalog :
114873
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
114873
Product Name :
BARK1 X073_pECIA14
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA14
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47051)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
SVADDQTAMLALAKSFNPPPSDWSSTTDFCKWSGVRCTG
GRVTTISLADKSLTGFIAPEISTLSELKSVSIQRNKLSG
TIPSFAKLSSLQEIYMDENNFVGVETGAFAGLTSLQILS
LSDNNNITTWSFPSELVDSTSLTTIYLDNTNIAGVLPDI
FDSLASLQNLRLSYNNITGVLPPSLGKSSIQNLWINNQD
LGMSGTIEVLSSMTSLSQAWLHKNHFFGPIPDLSKSENL
FDLQLRDNDLTGIVPPTLLTLASLKNISLDNNKFQGPLP
LFSPEVKVTIDHNVFCTTKAGQSCSPQVMTLLAVAGGLG
YPSMLAESWQGDDACSGWAYVSCDSAGKNVVTLNLGKHG
FTGFISPAIANLTSLKSLYLNGNDLTGVIPKELTFMTSL
QLIDVSNNNLRGEIPKFPATVKFSYKPGNALLGTNGGDG
SSPGTGGASGGPGGSSGGGGS
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence:
growth strain :
Prey vector BARK1 X073_pECIA14 should be used with bait vector BARK1 X073_pECIA2.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G23750, BAK1-Associating Receptor-Like Kinase 1, BARK1
geneAT3G23750
id821957
genbank_ids
mutation
nameAT3G23750
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA