This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MDIS2/MRH1 P1_pECIA14
catalog :
114839
citations: 1
Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher
product information
Catalog Number :
114839
Product Name :
MDIS2/MRH1 P1_pECIA14
article :
doi10.1038/nature25184
id28196350
pubmed_id29320478
bacterial resistance :
Ampicillin
cloning :
backbonepECIA14
backbone_mutation
backbone_originChris Garcia (Addgene plasmid # 47051)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
SLTSQGSALLKFRARVNSDPHGTLANWNVSGINDLCYWS
GVTCVDGKVQILDLSGYSLEGTLAPELSQLSDLRSLILS
RNHFSGGIPKEYGSFENLEVLDLRENDLSGQIPPELSNG
LSLKHLLLSGNKFSDDMRIKIVRLQSSYEVRLKKSPKLS
PLAVLGCINRKLGHCVSRNRIIQVKKVEAIVFRIKATSR
RFLKAFPSFLEETDIYKRRELLEETSNLAAEPAPSAPSP
SPGIITEASPRSSGSFPAVTNAKKRRPPLVPPVPSPDKG
STSPDISKNQPQDNKQSKGS
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence:
growth strain :
Prey vector MDIS2/MRH1 P1_pECIA14 should be used with bait vector MDIS2/MRH1 P1_pECIA2.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMetallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G18640, F28A21.50, F28A21_50, morphogenesis of root hair 1
geneMRH1
id827598
genbank_ids
mutation
nameAT4G18640
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
resistance markers :
4093
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA