SERK2 P5_pECIA14 | Addgene 114768 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

SERK2 P5_pECIA14

catalog :

114768

citations: 1

Reference
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed publisher

product information

Catalog Number :

114768

Product Name :

SERK2 P5_pECIA14

article :

doi	10.1038/nature25184
id	28196350
pubmed_id	29320478

bacterial resistance :

Ampicillin

cloning :

backbone

pECIA14

backbone_mutation

backbone_origin

Chris Garcia (Addgene plasmid # 47051)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

SSNMEGDALHSLRANLVDPNNVLQSWDPTLVNPCTWFHV
TCNNENSVIRVDLGNADLSGQLVPQLGQLKNLQYLELYS
NNITGPVPSDLGNLTNLVSLDLYLNSFTGPIPDSLGKLF
KLRFLRLNNNSLTGPIPMSLTNIMTLQVLDLSNNRLSGS
VPDNGSFSLFTPISFANNLDLCGPVTSRPCPGSPPFSPP
PPFIPPPIVPTPGGYS
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence:

growth strain :

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	Metallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1G34210, ATSERK2, F23M19.11, F23M19_11, somatic embryogenesis receptor-like kinase 2
gene	SERK2
id	840320

genbank_ids

mutation

name

AT1G34210

shRNA_sequence

size

species

3702

Arabidopsis thaliana