This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC19_T790_28bp
catalog :
114722
citations: 1
Reference
Hayward S, Lund P, Kang Q, Johnson Buck A, Tewari M, Walter N. Ultraspecific and Amplification-Free Quantification of Mutant DNA by Single-Molecule Kinetic Fingerprinting. J Am Chem Soc. 2018;140:11755-11762 pubmed publisher
product information
Catalog Number :
114722
Product Name :
pUC19_T790_28bp
article :
doi10.1021/jacs.8b06685
id28196347
pubmed_id30125495
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size2658
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Fragment contains 6 bp barcode TAGGAC at 3' end
growth strain :
28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ZraI
cloning_site_5MylI
promoter
sequencing_primer_3tgtggaattgtgagcgga
sequencing_primer_5TTTCCCAGTCACGACGTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
mutation
nameEGFR
shRNA_sequence
size28
species
9606
Homo sapiens
tags
resistance markers :
1046
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA