This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL3-TK-5UTR-BsmBI-Luciferase
catalog :
114670
citations: 2
Reference
Jiang F, Hedaya O, Khor E, Wu J, Auguste M, Yao P. RNA binding protein PRRC2B mediates translation of specific mRNAs and regulates cell cycle progression. Nucleic Acids Res. 2023;51:5831-5846 pubmed publisher
Jewer M, Lee L, Leibovitch M, Zhang G, Liu J, Findlay S, et al. Translational control of breast cancer plasticity. Nat Commun. 2020;11:2498 pubmed publisher
product information
Catalog Number :
114670
Product Name :
pGL3-TK-5UTR-BsmBI-Luciferase
article :
doi10.1038/s41467-020-16352-z
id28216439
pubmed_id32427827
bacterial resistance :
Ampicillin
cloning :
backbonepGL3
backbone_mutation
backbone_originPromega
backbone_size4895
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter
growth temp :
Can likely grow in other strains as well
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterlac promoter
sequencing_primer_3LucNrev
sequencing_primer_5RVprimer3
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameLacZ
shRNA_sequence
size459
species
tags
location
tagfor cloning 5' UTR-Luciferase fusions
resistance markers :
2776
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA