This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C
catalog :
114624
citations: 1
Reference
Warner J, Ruff K, Tan P, Lemke E, Pappu R, Lashuel H. Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths. J Am Chem Soc. 2017;139:14456-14469 pubmed publisher
product information
Catalog Number :
114624
Product Name :
pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C
article :
doi10.1021/jacs.7b06659
id28196339
pubmed_id28937758
bacterial resistance :
Ampicillin
cloning :
backbonepTWIN1
backbone_mutation
backbone_originNEB
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat
origin :
30
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T7-Term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
mutationA2C, P70C; 43 polyQ repeat
nameHuntingtin Exon1 fragment
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis6-Ssp DnaB intein
resistance markers :
1204
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA