This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHMMa.PH0999
catalog :
11453
citations: 1
Reference
Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim S. Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. Biochemistry. 2001;40:14166-72 pubmed
product information
Catalog Number :
11453
Product Name :
pHMMa.PH0999
article :
doi10.1021/bi0115479
id560
pubmed_id11714269
bacterial resistance :
Ampicillin
cloning :
backbonepHMMa
backbone_mutation
backbone_origin
backbone_size3188
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid pHMMa.PH0999 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genePH0999
id1443321
genbank_ids
BAA30096
PH0999
NP_142913
mutation
namepyrazinamidase
shRNA_sequence
size540
species
P. horikoshii
tags
resistance markers :
251
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA