pBABE hygro rat IRS-3 | Addgene 11450 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABE hygro rat IRS-3

catalog :

11450

citations: 1

Reference
Tsuruzoe K, Emkey R, Kriauciunas K, Ueki K, Kahn C. Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Mol Cell Biol. 2001;21:26-38 pubmed

product information

Catalog Number :

11450

Product Name :

pBABE hygro rat IRS-3

article :

doi	10.1128/MCB.21.1.26-38.2001
id	526
pubmed_id	11113178

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE hygro

backbone_mutation

backbone_origin

pBABE-hygro is available at Addgene

backbone_size

5558

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Rat IRS-3 was generated by PCR from the rat genomic DNA sequence. The intron was removed by PCR overlap extension using the following two pairs of primers: 5'-CGTGGATCCGCGATGAAGCCTGCAGGTACG-3' (sense) plus 5'-CTTGGGGGCTGAAACCCATGTTTGCTGGGCA-3' (antisense) and 5'-TGCCCAGCAAACATGGGTTTCAGCCCCCAAG-3' (sense) plus 5'-GCTGTCGACGTTCTAGAACTTGATGCTG-3' (antisense). BamHI and SalI sites were introduced at the 5' and 3' ends, respectively, of the coding sequence of IRS-3 by PCR. The subsequently generated coding sequence of rat IRS-3 was ligated into BamHI and SalI sites in pBABE-hygro.

origin :

pi :

alt_names

Insulin Receptor Substrate 3

IRS3

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Irs3
id	84021

genbank_ids

NM_032074

mutation

name

IRS-3

shRNA_sequence

size

1500

species

10116

Rattus norvegicus