AICSDP-64: FUS-mEGFP | Addgene 114410 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

AICSDP-64: FUS-mEGFP

catalog :

114410

citations: 1

Reference
Wienert B, Nguyen D, Guenther A, Feng S, Locke M, Wyman S, et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun. 2020;11:2109 pubmed publisher

product information

Catalog Number :

114410

Product Name :

AICSDP-64: FUS-mEGFP

article :

doi
id	28192802
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pUC57

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

Other

Donor Template

growth notes :

After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
ENLYFQGAAKFKETAAAKFERQHMDSGGGGSSGPSGSSS
LEVLFQGPLSSSGPSGS.
This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human FUS in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence:

growth strain :

Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
gene	FUS
id	2521

genbank_ids

mutation

homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-specific homozygous SNP(s)

name

FUS Homology Arms with linker-mEGFP

shRNA_sequence

size

2882

species

9606

Homo sapiens