This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSKB3.EG10680
catalog :
11436
citations: 1
Reference
Shin D, Choi I, Busso D, Jancarik J, Yokota H, Kim R, et al. Structure of OsmC from Escherichia coli: a salt-shock-induced protein. Acta Crystallogr D Biol Crystallogr. 2004;60:903-11 pubmed
product information
Catalog Number :
11436
Product Name :
pSKB3.EG10680
article :
doi10.1107/S0907444904005013
id554
pubmed_id15103136
bacterial resistance :
Kanamycin
cloning :
backbonepSKB3
backbone_mutation
backbone_origin
backbone_size5386
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesECK1476, JW1477
geneosmC
id946043
genbank_ids
AAC74555
NP_415999
EG10680
mutation
nameOsmC
shRNA_sequence
size429
species
E. coli
tags
resistance markers :
251
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA