This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCLXEBR-pTF-kRasV12
catalog :
114318
citations: 1
Reference
Chui M, Shih I. Oncogenic BRAF and KRAS mutations in endosalpingiosis. J Pathol. 2020;250:148-158 pubmed publisher
product information
Catalog Number :
114318
Product Name :
pCLXEBR-pTF-kRasV12
article :
doi
id28196292
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCLXEBR65
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. Subcloned from Addgene Plasmid #31200. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
growth strain :
TET-inducible expression of the V12-mutant form of the ki-Ras protein
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationG12V oncogenic mutation. Constitutively active K-ras protein.
nameRas
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
767
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA