This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSKB3.MPN625
catalog :
11430
citations: 1
Reference
Choi I, Shin D, Brandsen J, Jancarik J, Busso D, Yokota H, et al. Crystal structure of a stress inducible protein from Mycoplasma pneumoniae at 2.85 A resolution. J Struct Funct Genomics. 2003;4:31-4 pubmed
product information
Catalog Number :
11430
Product Name :
pSKB3.MPN625
article :
doi10.1023/A:1024625122089
id550
pubmed_id12943365
bacterial resistance :
Kanamycin
cloning :
backbonepSKB3
backbone_mutation
backbone_origin
backbone_size5386
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid pSKB3.MPN625 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
origin :
37
pi :
alt_names
stress inducible protein
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC12_orf141
geneMPN625
id877343
genbank_ids
AAB95865
NP_110314
MPN625
mutation
nameperoxiredoxin
shRNA_sequence
size423
species
M. pneumoniae
tags
resistance markers :
251
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA