This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSKB3.TM1777
catalog :
11421
citations: 1
Reference
Shin D, Nguyen H, Jancarik J, Yokota H, Kim R, Kim S. Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain. Biochemistry. 2003;42:13429-37 pubmed publisher
product information
Catalog Number :
11421
Product Name :
pSKB3.TM1777
article :
doi10.1021/bi035118h
id542
pubmed_id14621988
bacterial resistance :
Kanamycin
cloning :
backbonepSKB3
backbone_mutation
backbone_origin
backbone_size5386
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid pSKB3.TM1777 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genenusA
id897087
genbank_ids
AAD36840
NP_229574
TM1777
mutation
nameNusA
shRNA_sequence
size1032
species
32644
Other
T. maritima
tags
locationN terminal on backbone
tag6x His
locationN terminal on backbone
tagTEV protease site
resistance markers :
251
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA