This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET21a.TM1363
catalog :
11420
citations: 1
Reference
Shin D, Brandsen J, Jancarik J, Yokota H, Kim R, Kim S. Structural analyses of peptide release factor 1 from Thermotoga maritima reveal domain flexibility required for its interaction with the ribosome. J Mol Biol. 2004;341:227-39 pubmed publisher
product information
Catalog Number :
11420
Product Name :
pET21a.TM1363
article :
doi10.1016/j.jmb.2004.05.055
id540
pubmed_id15312775
bacterial resistance :
Ampicillin
cloning :
backbonepET21a
backbone_mutation
backbone_originNovagen
backbone_size5443
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please note that the plasmid pET21a.TM1363 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
origin :
37
pi :
alt_names
prfA
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneprfA
id898117
genbank_ids
AAD36433
NP_229164
TM1363
mutation
namepeptide release factor I
shRNA_sequence
size1026
species
Thermotoga maritima
tags
resistance markers :
251
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA